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> F(ab')2 Rat IgM (mu chain) antibody (RPE)

F(ab')2 Rat IgM (mu chain) antibody (RPE)

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Référence orb348349-1ml

Conditionnement : 1ml

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F(ab')2 Rat IgM (mu chain) antibody (RPE)

F(ab')2 Rat IgM (mu chain) antibody (RPE)

Catalog Number: orb348349

Catalog Numberorb348349
CategoryAntibodies
DescriptionF(ab')2 Rat IgM (mu chain) antibody (RPE)
Species/HostGoat
ClonalityPolyclonal
Tested applicationsFC, IF
ReactivityRat
IsotypeIgG F(ab')2
ImmunogenRat IgM whole molecule
Concentration0.5 mg/mL
Dilution rangeFC: 1:100 - 1:250, IF: 1:100 - 1:250
Form/AppearanceLyophilized
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgM, and Rat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other rat heavy or light chain proteins.
ConjugationRPE
StorageStore vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesGoat F(ab')2 Anti-Rat IgM (mu chain) Phycoerythrin
NoteFor research use only
Application notesF(ab')2 Anti-Rat IgM Antibody Phycoerythrin conjugation is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Expiration Date12 months from date of receipt.