FDG [Fluorescein di-beta-D-galactopyranoside] *CAS#: 17817-20-8*

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FDG [Fluorescein di-beta-D-galactopyranoside] *CAS#: 17817-20-8*

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Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.79001, 0.952
Although chromogenic assays of beta-galactosidase activity (i.e. X-Gal) are still widely used, the recent advance in fluorescence instrumentation has made fluorogenic substrates (such as FDG and MUG) be several orders of magnitude more sensitive. FDG is considered to be one of the most sensitive fluorogenic substrates available for detecting beta-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect ?-galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). FDG has been widely used for identifying LacZ-positive cells with fluorescence microscopy and flow cytometry. It is also used to detect beta-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FDG [Fluorescein di-beta-D-galactopyranoside] *CAS#: 17817-20-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM152.304 µL761.522 µL1.523 mL7.615 mL15.23 mL
5 mM30.461 µL152.304 µL304.609 µL1.523 mL3.046 mL
10 mM15.23 µL76.152 µL152.304 µL761.522 µL1.523 mL

Molarity calculator

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Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952



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