Glucokinase (GCK) Mouse Monoclonal Antibody [Clone ID: OTI3E3]
CAT#: CF500459
Carrier-free (BSA/glycerol-free) GCK mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
Formulation: Standard
Specifications
| Product Data | |
| Clone Name | OTI3E3 |
| Applications | IF, IHC, WB |
| Recommended Dilution | WB 1:500, IHC 1:50, IF 1:50 |
| Reactivities | Human, Mouse, Rat |
| Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human GCK (NP_000153) produced in HEK293T cell. |
| Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Predicted Protein Size | 52 kDa |
| Gene Name | glucokinase |
| Database Link | |
| Background | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq] |
| Synonyms | FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2 |
| Reference Data | |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus |
Documents
| Product Manuals |
| FAQs |
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| SDS |
Resources
| Antibody Resources |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| TA500459 | GCK mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) | 447,00 EUR | |
| TA500459AM | GCK mouse monoclonal antibody, clone 3E3, Biotinylated | 509,00 EUR | |
| TA500459BM | GCK mouse monoclonal antibody, clone 3E3, HRP conjugated | 509,00 EUR | |
| TA500459S | GCK mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) | 200,00 EUR |