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> GSK-3beta Antibody

GSK-3beta Antibody

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Référence ASM10424-A680-100

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  • ICC/IF - GSK-3beta Antibody ASM10424
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - GSK-3beta Antibody ASM10424
    Western blot analysis of Human Cell line lysates showing detection of GSK3 Beta protein using Rabbit Anti-GSK3 Beta Polyclonal Antibody (ASM10424). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-GSK3 Beta Polyclonal Antibody (ASM10424) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
  • ICC/IF - GSK-3beta Antibody ASM10424
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession P49841
Other Accession NP_001139628.1
Host Rabbit
Reactivity Human, Mouse, Rat, Bovine
Clonality Polyclonal
Description Rabbit Anti-Human GSK-3beta Polyclonal
Target/Specificity Detects ~47kDa.
Other Names Glycogen Synthase kinase 3 beta Antibody, GSK 3 beta Antibody, GSK 3B Antibody, GSK-3 beta Antibody, GSK3B Antibody, GSK3B_Human Antibody, GSK3beta isoform Antibody
Immunogen Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | Cell Membrane