| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | IHC, WB |
| Purification | Stabilized antiserum |
| Reactivity | Human, Mouse |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Synthetic peptide of human keratin K74 (formerly also des-ignated keratin K6irs4; C-GKSTPASIP ARKATR), coupled to KLH |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | Q7RTS7 (Human), Q6IFZ9 (Mouse) |
| Synonym | Keratin, type II cytoskeletal 74, Cytokeratin-74, CK-74, Keratin-5c, K5C, Keratin-74, K74, Type II inner root sheath-specific keratin-K6irs4, Type-II keratin Kb37, KRT74, K6IRS4, KB37, KRT5C, KRT6IRS4 |
| Note | Centrifuge prior to opening |
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100-1:200 |
| Western Blot (WB) | 1:2,000 |
The antiserum stains specifically the Huxley layer of the inner root sheath (irs) of the hair follicle (from matrix to "end" of irs).
Although there is a considerable sequence homology of keratin K74 polypeptide to keratins K5 and K6, no cross-reactivity was observed with Western blotting and immunofluorescence microscopy.