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> HDEL antibody

HDEL antibody

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Référence orb147722-100ug

Conditionnement : 100ug

Marque : Biorbyt

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HDEL antibody

HDEL antibody

Catalog Number: orb147722

Catalog Numberorb147722
CategoryAntibodies
DescriptionMouse monoclonal to HDEL (FITC). HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S. cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat..
Species/HostMouse
ClonalityMonoclonal
Clone Number20000000
Tested applicationsICC, WB
ReactivityDrosophila, Plant, Saccharomyces, Yeast
IsotypeIgG2b
ImmunogenRaised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Concentration1 mg/ml
Dilution rangeWB (1:1000), ICC/IF (1:100)
ConjugationFITC
MW78kDa
TargetHDEL
StorageConjugated antibodies should be stored at 4°C
Buffer/PreservativesPBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Alternative namesH-D-E-L (his-asp-glu-leu) antibody, endoplasmic re
NoteFor research use only
Application notes1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Expiration Date12 months from date of receipt.