Hexokinase 1, NT (Hexokinase-1, Hexokinase Type I, HEX1, HK I, HKI, HK1, HXK1, Brain Form Hexokinase)

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Western Blot: 1:1000-1:2000
Immunohistochemistry (Paraffin, Leica): 1:500
Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Pab

IgG

rabbit

human

As Reported

Supplied as a liquid in PBS, 0.09% sodium azide.

Purified by ammonium sulfate precipitation.

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 78-108 in the N-terminal region of human HK1.

Recognizes human HK1. Species Crossreactivity

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.