Hexokinase 1, NT (Hexokinase-1, Hexokinase Type I, HEX1, HK I, HKI, HK1, HXK1, Brain Form Hexokinase) (Azide free) (HRP)
Référence H2035-25N-HRP-200ul
Conditionnement : 200ul
Marque : US Biological
Host
rabbitConjugate
HRPIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBShipping Temp
Blue IceStorage Temp
-20°CHexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Product Type
Pab
Isotype
IgG
Host
rabbit
Source
human
Concentration
As Reported
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by Protein A/G affinity chromatography.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 78-108 in the N-terminal region of human HK1.
Specificity
Recognizes human HK1. Species Crossreactivity
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.