Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> HIF1 alpha Antibody

HIF1 alpha Antibody

Imprimer Imprimer
Identifiez-vous

Référence ASM10128-A680-100

Conditionnement : Onrequest

Marque : Abcepta

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

 
  • IHC - HIF1 alpha Antibody ASM10128
    Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 (ASM10128). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody (ASM10128) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis. .
  • WB - HIF1 alpha Antibody ASM10128
    Western Blot analysis of Human HeLa cell lysates showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 (ASM10128). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody (ASM10128) at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, E
Primary Accession Q61221
Other Accession NP_034561.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat, Bovine
Clonality Monoclonal
Description Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1
Target/Specificity Detects ~116kDa. Specific for HIF1Alpha.
Other Names ARNT interacting protein Antibody, HIF1A Antibody, Hypoxia inducible factor 1 alpha Antibody, MOP1 Antibody, PASD8 Antibody
Clone Names ESEE122
Immunogen Recombinant fragment corresponding to amino acids 329-530
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus