HSP40, YDJ1 antibody
Référence orb147329-100ug
Conditionnement : 100ug
Marque : Biorbyt
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HSP40, YDJ1 antibody
Catalog Number: orb147329
| Catalog Number | orb147329 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to Hsp40, YDJ1 (APC). Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. , type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins . The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 1G10.H8 |
| Tested applications | ELISA, IP, WB |
| Reactivity | Yeast |
| Isotype | IgG1 Kappa |
| Immunogen | Full length protein yeast HSP40 (YDJ1) |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:2000) |
| Conjugation | APC |
| MW | 40kDa |
| Target | HSP40 |
| Entrez | 855661 |
| UniProt ID | P25491 |
| NCBI | NP_014335.1 |
| Storage | Conjugated antibodies should be stored at 4°C |
| Buffer/Preservatives | 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
| Alternative names | DNAJA2 antibody, CPR3 antibody, HIRIP4 antibody, D |
| Note | For research use only |
| Application notes | 0.5 µg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |