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> HSP70 Antibody

HSP70 Antibody

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Référence ASM10022-A565-200

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  • ICC/IF - HSP70 Antibody ASM10022
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1.86 (ASM10022). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (ASM10022) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.
  • WB - HSP70 Antibody ASM10022
    Western Blot analysis of Human HEK293 cell lysate showing detection of ~70 kDa Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 1.86 (ASM10022). Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (ASM10022) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, E
Primary Accession Q27975
Other Accession NP_776975.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat, Pig, Bovine
Clonality Monoclonal
Description Mouse Anti-Bovine HSP70 Monoclonal IgG1
Target/Specificity Detects 70kDa. Does not cross react with HSC70.
Other Names HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
Clone Names 1.86
Immunogen Bovine HSP70
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-113 was sufficient for detection of HSP70 in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm