Human CTSB (Cathepsin B) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	CTSB
Gene ID:	1508
Uniprot ID:	CATB_HUMAN
Specificity:	This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
Function	Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.
Protein Name	Cathepsin B App Secretase Apps Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1679131 Reactome: R-HSA-2022090 Reactome: R-HSA-2132295 Reactome: R-HSA-6798695
Cellular Localisation	Lysosome Melanosome Secreted Extracellular Space Apical Cell Membrane Peripheral Membrane Protein Extracellular Side Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells
Alternative CLIA Names	Cathepsin B CLIA kit App Secretase CLIA kit Apps CLIA kit Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain CLIA kit CTSB CLIA kit CPSB CLIA kit
Specificity	This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	98-111	104
EDTA plasma(n=8)	101-116	108
Cell culture media(n=8)	100-114	106

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	100.33	277.70	727.18	92.77	304.35	793.10
Standard deviation	8.14	28.52	62.46	11.96	22.92	89.54
CV (%)	8.11	10.27	8.59	12.89	7.53	11.29

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance