General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This human CTSV kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | CTSV |
| Gene ID: | 1515 |
| Uniprot ID: | CATL2_HUMAN |
| Specificity: | This kit recognizes Human CTSV in samples. No significant cross-reactivity or interference between Human CTSV and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Tissue Specificity | Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin. |
| Function | Cysteine protease. May have an important role in corneal physiology. |
| Protein Name | Cathepsin L2Cathepsin UCathepsin V |
| Database Links | Reactome: R-HSA-1236977Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-1679131Reactome: R-HSA-2022090Reactome: R-HSA-2132295Reactome: R-HSA-8939242 |
| Cellular Localisation | Lysosome |
| Alternative CLIA Names | Cathepsin L2 CLIA kitCathepsin U CLIA kitCathepsin V CLIA kitCTSV CLIA kitCATL2 CLIA kitCTSL2 CLIA kitCTSU CLIA kitUNQ268 CLIA kitPRO305 CLIA kit |
| Specificity | This kit recognizes Human CTSV in samples. No significant cross-reactivity or interference between Human CTSV and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 98-114 | 105 |
| EDTA plasma(n=8) | 96-112 | 102 |
| Cell culture media(n=8) | 97-111 | 105 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.25 | 109.20 | 369.91 | 46.37 | 100.73 | 384.17 |
| Standard deviation | 6.16 | 11.50 | 29.04 | 3.83 | 7.50 | 27.31 |
| CV (%) | 12.26 | 10.53 | 7.85 | 8.26 | 7.45 | 7.11 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance