Human DPP4 (Dipeptidyl peptidase 4) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	DPP4
Gene ID:	1803
Uniprot ID:	DPP4_HUMAN
Specificity:	This kit recognizes Human DPP4 in samples. No significant cross-reactivity or interference between Human DPP4 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
Post Translational Modifications	The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing. N- and O-Glycosylated. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
Function	Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.
Protein Name	Dipeptidyl Peptidase 4 Adabp Adenosine Deaminase Complexing Protein 2 Adcp-2 Dipeptidyl Peptidase Iv Dpp Iv T-Cell Activation Antigen Cd26 Tp103 Cd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane Form Dipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble Form Dipeptidyl Peptidase Iv Soluble Form
Database Links	Reactome: R-HSA-381771 Reactome: R-HSA-400511
Cellular Localisation	Dipeptidyl Peptidase 4 Soluble Form: Secreted Detected In The Serum And The Seminal Fluid Cell Membrane Single-Pass Type Ii Membrane Protein Apical Cell Membrane Cell Projection Invadopodium Membrane Lamellipodium Membrane Cell Junction Membrane Raft Translocated To The Apical Membrane Through The Concerted Action Of N- And O-Glycans And Its Association With Lipid Microdomains Containing Cholesterol And Sphingolipids Redistributed To Membrane Rafts In T-Cell In An Interleukin-12-Dependent Activation Its Interaction With Cav1 Is Necessary For Its Translocation To Membrane Rafts Colocalized With Ptprc In Membrane Rafts Colocalized With Fap In Invadopodia And Lamellipodia Of Migratory Activated Endothelial Cells In Collagenous Matrix Colocalized With Fap On Endothelial Cells Of Capillary-Like Microvessels But Not Large Vessels Within Invasive Breast Ductal Carcinoma Colocalized With Ada At The Cell Junction In Lymphocyte-Epithelial Cell Adhesion Colocalized With Igf2r In Internalized Cytoplasmic Vesicles Adjacent To The Cell Surface
Alternative CLIA Names	Dipeptidyl Peptidase 4 CLIA kit Adabp CLIA kit Adenosine Deaminase Complexing Protein 2 CLIA kit Adcp-2 CLIA kit Dipeptidyl Peptidase Iv CLIA kit Dpp Iv CLIA kit T-Cell Activation Antigen Cd26 CLIA kit Tp103 CLIA kit Cd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane Form CLIA kit Dipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble Form CLIA kit Dipeptidyl Peptidase Iv Soluble Form CLIA kit DPP4 CLIA kit ADCP2 CLIA kit CD26 CLIA kit
Specificity	This kit recognizes Human DPP4 in samples. No significant cross-reactivity or interference between Human DPP4 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-111	102
EDTA plasma(n=8)	95-109	102
Cell culture media(n=8)	88-101	94

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	237.67	673.60	2467.34	224.47	722.14	2334.61
Standard deviation	30.28	50.39	201.09	25.77	56.98	157.35
CV (%)	12.74	7.48	8.15	11.48	7.89	6.74

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance