Specifications

Product Data
Clone Name	NI 307
Applications	ELISA, IF, IP, WB
Recommended Dilution	To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE. General Recommended Dilutions: Histochemical Use: 1/50-1/200.
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Highly purified polyclonal IgE isolated from Human serum.
Specificity	The reactivity of the antiserum is restricted to an isotype specific determinant of the IgE molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation	PBS, pH 7.2 without preservatives or foreign proteins Label: FITC State: Lyophilized purified Ig fraction Label: Fluorescein Isothiocyanate Absorption emission: 492 nm / 515 nm Molar radio: Fluorochrome/IgG ~3.8
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water
Concentration	0.4 mg/ml
Conjugation	FITC
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.