Human (lambda chain) Antibody Peroxidase Conjugated

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommended Dilution	WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:50,000
Reactivities	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Anti-Human lambda chain was produced by repeated immunization with human lambda light chain in goat.
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration	1.0 mg/ml - lot specific
Conjugation	HRP
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Note	Anti-Human λ (lambda chain) Antibody peroxidase conjugated has been tested by ELISA and western blot and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:16,000 to 1:64,000 is suggested for this product. This secondary peroxidase conjugated antibody anti-Human lambda chain is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.