Human UCHL1 (Ubiquitin carboxyl-terminal hydrolase isozyme L1) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	UCHL1
Gene ID:	7345
Uniprot ID:	UCHL1_HUMAN
Specificity:	This kit recognizes Human UCHL1 in samples. No significant cross-reactivity or interference between Human UCHL1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Post Translational Modifications	O-glycosylated.
Function	Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis. Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators. Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation.
Protein Name	Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 Uch-L1 Neuron Cytoplasmic Protein 9.5 Pgp 9.5 Pgp9.5 Ubiquitin Thioesterase L1
Database Links	Reactome: R-HSA-5689603
Cellular Localisation	Cytoplasm Endoplasmic Reticulum Membrane Lipid-Anchor About 30% Of Total Uchl1 Is Associated With Membranes In Brain Localizes Near And/Or Within Mitochondria To Potentially Interact With Mitochondrial Proteins
Alternative CLIA Names	Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 CLIA kit Uch-L1 CLIA kit Neuron Cytoplasmic Protein 9.5 CLIA kit Pgp 9.5 CLIA kit Pgp9.5 CLIA kit Ubiquitin Thioesterase L1 CLIA kit UCHL1 CLIA kit
Specificity	This kit recognizes Human UCHL1 in samples. No significant cross-reactivity or interference between Human UCHL1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-105	96
EDTA plasma(n=8)	98-114	107
Cell culture media(n=8)	90-101	96

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	47.88	92.40	378.30	49.47	96.38	373.46
Standard deviation	6.21	7.95	25.27	6.01	11.35	24.72
CV (%)	12.97	8.60	6.68	12.15	11.78	6.62

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance