IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc Antibody (TRITC)

Référence LS-C346691-1

Conditionnement : 1ml

Marque : LSBio

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Overview

Antibody:
IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc Goat anti-Rat Polyclonal (TRITC) Antibody
Application:
IHC, IHC-Fr, ICC, IF, ELISA
Reactivity:
Rat
Format:
Tetramethyl Rhodamine Iso-Thiocyanate, Unmodified

Specifications

Description
IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc antibody LS-C346691 is a TRITC-conjugated goat polyclonal antibody to rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc. Validated for ELISA, ICC, IF and IHC.
Target
Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Tetramethyl Rhodamine Iso-Thiocyanate. Also available Unconjugated or conjugated with FITC, HRP, Biotin.
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgG, IgA and IgM, Fc specific.
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Immunofluorescence
  • ELISA
  • Applications tested for the base form of this product only
Usage
The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the rat immunoglobulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

tech@hexabiogen.com