IgE (Biotin)

Suitable for use in ELISA and Western Blot. Other applications have not been tested.

ELISA: 500ng/ml as detecting antibody
Optimal working dilutions to be determined by researcher.

Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Mab

IgG1

3H2277 (MARE-1)

mouse

rat

~1mg/ml

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.

Purified by affinity chromatography from tissue culture supernatant.

Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.

Recognizes the epsilon chain of rat IgE and does not crossreact with other classes of rat immunoglobulin. Binds to rat IgE with an avidity of 4x10e9M-1

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.