IgE, Heavy Chain (HRP)

Suitable for use in ELISA and Western Blot. Other applications have not been tested

Optimal working dilutions to be determined by researcher.

Spleen cells from immunized BALB/c mice fused with mouse SP2/0 myeloma cells

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Mab

IgG1

3H2277 (MARE-1)

mouse

rat

As Reported

Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).

Purified by Protein G affinity chromatography from tissue culture supernatant.

Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.

Recognizes the epsilon chain of rat IgE. Does not cross-react with other classes of rat immunoglobulin.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.