IgE (HRP)
Référence I1900-30A-500ug
Conditionnement : 500ug
Marque : US Biological
Host
mouseConjugate
HRPIsotype
IgG1Clone Number
3H2277 (MARE-1)Grade
Affinity PurifiedApplications
E WBShipping Temp
Blue IceStorage Temp
-20°CApplications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Affinity Constant:
4x10e9M-1
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Mab
Isotype
IgG1
Clone No
3H2277 (MARE-1)
Host
mouse
Source
rat
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Purity
Purified by affinity chromatography from tissue culture supernatent
Immunogen
Rat IgE myeloma protein (IR162, IR1016, IR2 ,IR410)
Specificity
Recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.