IgG Fab Antibody (TRITC)

Rat IgG Fab

Sheep

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Tetramethyl Rhodamine Iso-Thiocyanate. Also available Unconjugated or conjugated with Biotin, FITC, HRP.

Purified IgG

Unmodified

Purified Fab of normal IgG isolated form rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat Fab of IgG.

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled rat serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab. Direct staining of fixed cell and tissue substrates. to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.