Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> IgM (AP)

IgM (AP)

Imprimer Imprimer
Identifiez-vous

Référence I1905-01U-1mg

Conditionnement : 1mg

Marque : US Biological

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Host
goat

Conjugate
AP

Isotype
IgG

Grade
Affinity Purified

Applications
E IHC WB

Shipping Temp
Blue Ice

Storage Temp
4°C Do Not Freeze

Applications:
Suitable for immunoblot, ELISA, immunohistochemistry and other phosphatase-antibody based enzymatic assays
Other applications not tested.
Recommended Dilutions:
ELISA: 1:500-1:2000 (Coat with1ug of Rat IgM).
Optimal dilutions should be determined by the researcher.
Label:
Alkaline Phosphatase (Calf Intestine)
Molecular Weight:
140kD
Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Type
Pab
Isotype
IgG
Host
goat
Source
rat
Concentration
~1mg/ml (UV280)
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Purity
Purified by immunoaffinity chromatography.
Immunogen
Rat IgM heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgM and Rat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other Rat or human heavy or light chain isotypes.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.