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> IL4 antibody [MP4-25D2] (APC)

IL4 antibody [MP4-25D2] (APC)

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Identifiez-vous

Référence GTX01492-07-100test

Conditionnement : 100test

Marque : Genetex

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Téléphone : +1 850 650 7790

Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MP4-25D2

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Rhesus Monkey
Package
100 test
Datasheet File
View product citations for antibody GTX01492-07 on CiteAb
PRODUCT

Summary

The MP4-25D2 antibody is specific for human IL-4, a pleiotropic cytokine that Th2 cell differentiation, B cell proliferation and differentiation, and B cell class switching to IgE. MP4-25D2 is a neutralizing antibody and is reported to cross react with rhesus monkey IL-4.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.0375 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.0075 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 4 , BCGF1 , BSF1 , IL4 , Interleukin 4

Cellular Localization

Secreted

Background

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07

GTX01492-07 FACS Image

FACS analysis of human peripheral blood lymphocytes (with PMA and Ionomycin stimulation) using GTX01492-07 IL4 antibody [MP4-25D2] (APC).
Right panel : co-stained with IL4 antibody [MP4-25D2] (APC) and Human CD3 antibody (PE)
Left panel : co-stained with isotype control and Human CD3 antibody (PE)
antibody amount : 0.0375 μg (5 μl)

Clone Reference

1995; Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies.
1995; Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells.
1992; Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
1989; Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4.

REVIEW

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Clone Reference
1995; Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies.
1995; Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells.
1992; Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
1989; Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4.
SDS
Sodium Azide.pdf