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> INPP5F Antibody

INPP5F Antibody

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Référence ASM10296-P594-100

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  • ICC/IF - INPP5F Antibody ASM10296
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 (ASM10296). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody (ASM10296) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Endosome, Endosome Membrane, Membrane, Golgi Apparatus, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) INPP5F Antibody (D) Composite.
  • WB - INPP5F Antibody ASM10296
    Western Blot analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of ~100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 (ASM10296). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody (ASM10296) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q01968
Other Accession NP_000267.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human INPP5F Monoclonal IgG1
Target/Specificity Detects ~100kDa. Cross-reacts with INPP5b.
Other Names EC 3.1.3.36 Antibody, Inositol polyphosphate 5 phosphatase OCRL 1 Antibody, Inositol polyphosphate 5 phosphatase OCRL1 Antibody, Inositol polyphosphate 5-phosphatase OCRL-1 Antibody, INPP5F Antibody, LOCR Antibody, Lowe oculocerebrorenal syndrome protein Antibody, NPHL2 Antibody, OCRL 1 Antibody, OCRL Antibody, OCRL_HUMAN Antibody, OCRL1 Antibody, Oculocerebrorenal syndrome of Lowe Antibody, Phosphatidylinositol polyphosphate 5 phosphatase Antibody
Clone Names S166A-26
Immunogen Fusion protein amino acids 1-901 (full-length) of human INPP5F. Rat: 93% identity (845/904 amino acids identical). Mouse: 91% identity (824/901 amino acids identical) ~50% identity with INPP5b.
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-464 was sufficient for detection of INPP5F in 20 µg of COS cells transiently transformed with GFP-tagged OCLR lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasmic Vesicle | Endosome | Endosome Membrane | Membrane | Golgi Apparatus