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> MDC1 Antibody

MDC1 Antibody

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Référence ASM10145-ALP-100

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  • ICC/IF - MDC1 Antibody ASM10145
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (ASM10145). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (ASM10145) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
  • WB - MDC1 Antibody ASM10145
    Western Blot analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (ASM10145). Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (ASM10145) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.
  • WB - MDC1 Antibody ASM10145
    Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (ASM10145). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (ASM10145) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q5PSV9
Other Accession NP_001010833.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Chimpanzee, Bovine
Clonality Monoclonal
Description Mouse Anti-Mouse MDC1 Monoclonal IgG1
Target/Specificity Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.
Other Names Nuclear factor with BRCT domains1 Antibody, mediator of DNA damage checkpoint 1 Antibody
Clone Names P2B11
Immunogen GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization Nucleus