Specifications

Product Data
Clone Name	2G5
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of Neat-1/5 diluted antibody to label 10e6 cells in 100 µl.
Reactivities	Bovine, Guinea Pig, Hamster, Human, Mouse, Porcine, Rat
Host	Mouse
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Purified H-2Kb and H-2Db MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
Specificity	This antibody recognises a monomorphic epitope on the MHC class I antigen. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some Canine samples suggesting that this antibody may recognise a polymorphic epitope of Canine MHC class 1.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Background	MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Synonyms	MHC Class 1, HLA Class 1
Reference Data