Mouse HGF (Hepatocyte Growth Factor) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	Hgf
Gene ID:	15234
Uniprot ID:	HGF_MOUSE
Specificity:	This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.
Function	Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.
Protein Name	Hepatocyte Growth Factor Hepatopoietin-A Scatter Factor Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain
Database Links	Reactome: R-MMU-114608 Reactome: -MMU-1257604 Reactome: -MMU-5673001 Reactome: -MMU-6806942 Reactome: -MMU-6807004 Reactome: -MMU-6811558 Reactome: -MMU-8851805 Reactome: -MMU-8851907 Reactome: -MMU-8865999 Reactome: -MMU-8874081 Reactome: -MMU-8875513 Reactome: -MMU-8875555 Reactome: -MMU-8875656 Reactome: -MMU-8875791 Reactome: -MMU-9734091
Alternative CLIA Names	Hepatocyte Growth Factor CLIA kit Hepatopoietin-A CLIA kit Scatter Factor CLIA kit Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain CLIA kit Hgf CLIA kit
Specificity	This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	98-109	104
EDTA plasma(n=8)	87-100	93
Cell culture media(n=8)	87-100	93

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	93.38	256.01	937.95	86.51	236.30	958.90
Standard deviation	8.11	27.98	88.45	10.75	21.13	59.36
CV (%)	8.68	10.93	9.43	12.43	8.94	6.19

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance