Mouse IgA (+IgG +IgM) Goat Polyclonal Antibody

Specifications

Product Data
Recommended Dilution	Suitable for Immunoblotting (1/500-1/3,000 for Western blot), ELISA (1/100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/250-1/1,500) as well as other Peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Mouse IgG, IgA and IgM whole molecules.
Specificity	This product was prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all mouse immunoglobulin isotypes and chain combinations.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerosal as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase Conjugation Chemistry: periodate
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity Chromatography.
Conjugation	HRP
Storage	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.