Mouse IRS1 (Insulin Receptor Substrate 1) Sandwich ELISA Kit Ready-To-Use E0016449)

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	Irs1
Gene ID:	16367
Uniprot ID:	IRS1_MOUSE
Immunogen Region:	Ready-To-Use
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Expressed in osteoblasts, but not in osteoclasts.
Post Translational Modifications	Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-891 is required for GRB2-binding. Phosphorylated by ALK. Phosphorylated at Ser-265, Ser-302, Ser-632 and Ser-1097 by RPS6KB1.phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-608 by TNS2 under anabolic conditions.dephosphorylation results in the proteasomal degradation of IRS1. Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).
Function	May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Protein Name	Insulin Receptor Substrate 1 Irs-1
Database Links	Reactome: R-MMU-109704 Reactome: -MMU-112399 Reactome: -MMU-112412 Reactome: -MMU-1257604 Reactome: -MMU-1266695 Reactome: -MMU-198203 Reactome: -MMU-201556 Reactome: -MMU-2428928 Reactome: -MMU-5673001 Reactome: -MMU-6811558 Reactome: -MMU-74713 Reactome: -MMU-74749
Cellular Localisation
Alternative ELISA Names	Insulin Receptor Substrate 1 ELISA kit Irs-1 ELISA kit Irs1 ELISA kit Irs-1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance