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Mouse Monoclonal Anti-PGP9.5 / UchL1 (pan-Neuronal Marker) [Clone UCHL1/775]
Référence NB-36-00839-P0
Conditionnement : 20ug
Marque : Neo Biotech
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Printable Datasheet
PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody [UCHL1/775]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded Rat Testis stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).
Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).
Western Blot Analysis of human brain tissue lysate using Pgp9.5 Monoclonal Antibody (UCHL1/775).
SDS-PAGE Analysis of Purified Pgp9.5 MAb (UCHL1/775). Confirmation of Purity and Integrity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Dog, Human, Rat
Research Areas
Neuroscience
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum membrane
Gene Name
UCHL1
Positive Control
Cerebellum.
Immunogen
Recombinant human UCHL1 protein
Alternate Names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1
Functions
- Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865).
Key References
- Wilkinson, K.D., et al. 1989. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science 246: 670-672.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/15815621/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/2163617/
- https://pubmed.ncbi.nlm.nih.gov/14722078/
- https://pubmed.ncbi.nlm.nih.gov/2947814/
- https://pubmed.ncbi.nlm.nih.gov/9774100/
- https://pubmed.ncbi.nlm.nih.gov/1849484/
- https://pubmed.ncbi.nlm.nih.gov/1286667/
- https://pubmed.ncbi.nlm.nih.gov/8639624/
- https://pubmed.ncbi.nlm.nih.gov/9790970/
- https://pubmed.ncbi.nlm.nih.gov/12408865/
- https://pubmed.ncbi.nlm.nih.gov/12082530/
- https://pubmed.ncbi.nlm.nih.gov/16475834/
- https://pubmed.ncbi.nlm.nih.gov/19261853/
- https://pubmed.ncbi.nlm.nih.gov/19608861/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
- https://pubmed.ncbi.nlm.nih.gov/23359680/
- https://pubmed.ncbi.nlm.nih.gov/16537382/
- https://pubmed.ncbi.nlm.nih.gov/20439756/
- https://pubmed.ncbi.nlm.nih.gov/12705903/
- https://pubmed.ncbi.nlm.nih.gov/10454131/
- https://pubmed.ncbi.nlm.nih.gov/10203348/
- https://pubmed.ncbi.nlm.nih.gov/11027850/
- https://pubmed.ncbi.nlm.nih.gov/15048890/
- https://pubmed.ncbi.nlm.nih.gov/16450370/
- https://pubmed.ncbi.nlm.nih.gov/18411255/
- https://pubmed.ncbi.nlm.nih.gov/21268678/
- https://pubmed.ncbi.nlm.nih.gov/28007905/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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