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> NALCN Antibody

NALCN Antibody

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Référence ASM10251-P594-100

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  • ICC/IF - NALCN Antibody ASM10251
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 (ASM10251). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody (ASM10251) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NALCN Antibody (D) Composite.
  • WB - NALCN Antibody ASM10251
    Western Blot analysis of Rat Brain showing detection of ~200 kDa NALCN protein using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 (ASM10251). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody (ASM10251) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q6Q760
Other Accession NP_705894.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat NALCN Monoclonal IgG1
Target/Specificity Detects ~200kDa.
Other Names VGCNL1 Antibody, CanIon Antibody, Sodium leak channel non-selective protein Antibody, Voltage gated channel-like protein 1 Antibody, A530023G15Rik Antibody, bA430M15.1 Antibody, FLJ23913 Antibody, FLJ44659 Antibody, FLJ44764 Antibody, Four repeat voltage gated ion channel Antibody, MGC74524 Antibody, Putative 4 repeat voltage gated ion channel Antibody, Sodium leak channel non-selective protein Antibody
Clone Names S187-7
Immunogen Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-417 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane