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> Nav1.8 Antibody

Nav1.8 Antibody

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Référence ASM10218-P594-100

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  • ICC/IF - Nav1.8 Antibody ASM10218
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12 (ASM10218). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (ASM10218) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic staining and some dull nuclear staining.
  • WB - Nav1.8 Antibody ASM10218
    Western Blot analysis of Monkey COS transient cell lysate showing detection of Nav1.8 protein using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12 (ASM10218). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (ASM10218) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • IHC - Nav1.8 Antibody ASM10218
    Immunohistochemistry analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12 (ASM10218). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (ASM10218) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Heavy filaggrin-like staining, lower epidermal cells have some staining.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, AM
Primary Accession Q62968
Other Accession NP_058943.1
Host Mouse
Isotype IgG2a
Reactivity Human, Mouse, Rat, Monkey
Clonality Monoclonal
Description Mouse Anti-Rat Nav1.8 Monoclonal IgG2a
Target/Specificity Detects ~220kDa. No cross reactivity against other Nav channels.
Other Names mPN3 antibody, Peripheral nerve sodium channel 3 antibody, Pn3 (gene name) antibody, PN3 antibody, Scn10a antibody, Sensory neuron sodium channel antibody, Sns (gene name) antibody, SNS antibody, Sodium channel protein type 10 subunit alpha antibody, Sodium channel protein type X alpha subunit antibody, Voltage-gated sodium channel alpha subunit Nav1.8 antibody
Clone Names S134-12
Immunogen Fusion protein amino acids 1724-1956 of rat Nav1.8
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-342 was sufficient for detection of Nav1.8 in 10 µg of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane