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Datasheet Ni-IDA Resin allows rapid affinity purification of His-tagged proteins. His-tagged proteins bind to Ni2+ cations, which are immobilized on the Ni-IDA resin by 3 metal-chelating sites. After unbound proteins are washed away, the target proteins are recovered by gradient elution. It is suitable for both native and denatured protein purification. Specifications:
Research Articles on Ni-IDA Resin
- Resin: Cross-linked 6% agarose
- Ligand: IDA
- Shape: Sphere
- Pore Size: 90 µm
- Binding Capacity: 20-40 mg/ml wet gel
- Recommended Flow Rate: < 300 cm/h
- Highest Resisistance of Atmospheric Pressure: 0.3 MPa
- pH Stability: 2-14
| Target | Ni-IDA Resin |
| Storage | Store at 2-8 °C (with 20% ethanol) for up to 2 years. |
| Buffer | Note: Buffers are not included with this product. Equilibration Buffer for soluble proteins: 50 mM sodium phosphate buffer, 300 mM NaCl, 10 mM imidazole, 10 mM Tris-HCl, pH 8.0. Equilibration Buffer for inclusion bodies: 100 mM sodium phosphate buffer, 6 M GuHCl, 10 mM Tris-HCl, pH 8.0; or 100 mM sodium phosphate buffer, 8 M urea, 10 mM Tris-HCl, pH 8.0. |
| Availability | Shipped within 10-20 working days. |
| Note | This product is for research use only. |
| Directions for use | Preparing the Ni-IDA purification column:
To avoid blocking the column, samples should be centrifuged and filtered through a 0.45 µm filter before loading. Loading samples and washing:Load samples and wash with 5-10 bed volume of equilibration buffer, and collect the flow-through in a tube Elute:Elute proteins with imidazole or low pH buffer. Regeneration of Ni-IDA resin:
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