NOS2 Antibody (C-11) is an IgG₁ κ mouse monoclonal NOS2 antibody (also designated iNOS antibody or NOS2A antibody) that detects the NOS2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. NOS2 Antibody (C-11) is available as both the non-conjugated anti-NOS2 antibody form, as well as multiple conjugated forms of anti-NOS2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Epithelial autotoxicity of nitric oxide: role in the respiratory cytopathology of pertussis.  |  Heiss, LN., et al. 1994. Proc Natl Acad Sci U S A. 91: 267-70. PMID: 7506415
2. Requirement for transcription factor IRF-1 in NO synthase induction in macrophages.  |  Kamijo, R., et al. 1994. Science. 263: 1612-5. PMID: 7510419
3. Nitric oxide synthases: roles, tolls, and controls.  |  Nathan, C. and Xie, QW. 1994. Cell. 78: 915-8. PMID: 7522969
4. Nitric oxide synthase: aspects concerning structure and catalysis.  |  Marletta, MA. 1994. Cell. 78: 927-30. PMID: 7522970
5. Regulation of nitric oxide synthase activity in human immunodeficiency virus type 1 (HIV-1)-infected monocytes: implications for HIV-associated neurological disease.  |  Bukrinsky, MI., et al. 1995. J Exp Med. 181: 735-45. PMID: 7530762
6. NO at work.  |  Schmidt, HH. and Walter, U. 1994. Cell. 78: 919-25. PMID: 7923361
7. Nitric oxide is an important mediator for tumoricidal activity in vivo.  |  Farias-Eisner, R., et al. 1994. Proc Natl Acad Sci U S A. 91: 9407-11. PMID: 7937779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-iNOS Antibody (C-11) has 582 citations in a variety of scientific publications.

Primary antibodies like Anti-iNOS Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

L'anticorps pan NOS préféré est l'Anticorps pan NOS (NOS-3F7-B11 B5): sc-58399.