Oct-2 Antibody (PT2) is an IgG₁ κ mouse monoclonal Oct-2 antibody (also designated POU class 2 homeobox 2 (POU2F2) antibody, OCT2 antibody, POU domain homeobox-containing transcription factor 2F2 antibody, or octamer 5′-ATTTGCAT-3′ binding transcription factor antibody) that detects the Oct-2 protein of human origin by WB, IP, IF, IHC(P) and ELISA. Oct-2 Antibody (PT2) is available as both the non-conjugated anti-Oct-2 antibody form, as well as multiple conjugated forms of anti-Oct-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration.  |  Tomilin, A., et al. 2000. Cell. 103: 853-64. PMID: 11136971
2. A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.  |  Scheidereit, C., et al. 1988. Nature. 336: 551-7. PMID: 2904654
3. The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains.  |  Clerc, RG., et al. 1988. Genes Dev. 2: 1570-81. PMID: 3265124

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Oct-2 Antibody (PT2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Oct-2 Antibody (PT2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.