PACSIN3 (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3, SH3 Domain-containing Protein 6511, Endophilin I)

A member of the Endophilin family, Endophilin A1 is a protein containing SH3 domains, and has been implicated in the trafficking of clathrin-mediated synaptic vesicle endocytosis within the membrane. It was originally characterized as a presynaptically enriched protein that binds to endocytic proteins polyphosphoinositide synaptojanin and dynamin. Endophilin A1 has been implicated in endocytic vesicle budding when its function in cell-free systems and in living synapses has been disturbed. Along with the SH3 domain, Endophilin A1 contains a BAR domain in the N-terminal region, that has been found to induce and bind membrane curvature. This protein's expression is localized to the brain, most specifically in the frontal cortex. Markedly high expression is observed in fetal cerebellum.

Suitable for use in ELISA and Western Blot. Other applications not tested.

ELISA: 1:4000
Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Pab

IgG

goat

human

~0.5mg/ml

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purified by immunoaffinity chromatography.

Synthetic peptide corresponding to APEEDAGGEALGGS-C, from human PACSIN3, at N-terminal (NP_057307.2).

Recognizes human PACSIN3. Species sequence homology

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.