PCNA (PC10)
Référence sc-56
Conditionnement : 200µg/ml
Marque : Santa Cruz Biotechnology
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PCNA Antibody (PC10): sc-56. Fluorescent western blot analysis of PCNA expression in HCT-116 (A), Raji (B), HeLa (C), MOLT-4 (D), NIH/3T3 (E) and KNRK (F) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgG2a BP-CFL 647: sc-542738.
Anticorps PCNA (PC10): sc-56
- L'anticorps PCNA PC10 est un monoclonal IgG2a κ L'Anticorps PCNA, cité dans 2.910 publications, fourni en 200 µg/ml
- raised against rat PCNA made in the protein A expression vector pR1T2T
- L'Anticorps PCNA (PC10) est recommandé pour la détection de PCNA p36 protein expressed at high levels in proliferating cells d'origine mouse, rat, human, insect et S. pombe par WB, IP, IF, IHC(P) et FCM
- Anti-L'Anticorps PCNA (PC10) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
- aussi disponible conjugué à la biotine pour WB, IHC(P) et ELISA; et soit au TRITC ou Alexa Fluor® 405 pour IF, IHC(P) et FCM
- m-IgG Fc BP-HRP, 2a BP-HRP">m-IgG2a BP-HRP et m-IgGκ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps PCNA (PC10) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps PCNA (PC10) ().
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Information sur le Gène
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Research Information
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PCNA Antibody (PC10) is an IgG2a κ mouse monoclonal PCNA antibody (also designated ATLD2 antibody) that detects the PCNA protein of m, r, h, insect, S. pombe origin by WB, IP, IF, IHC(P) and FCM. PCNA Antibody (PC10) is available as both the non-conjugated anti-PCNA antibody form, as well as multiple conjugated forms of anti-PCNA antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The PCNA (proliferating cell nuclear antigen) protein is synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, the PCNA protein exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. Thus, in staining applications, the PCNA Antibody exhibits nuclear staining. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives. Thus, only the synthesizing form is detectable by the PCNA antibody using this method.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- The contribution of E2F-regulated transcription to Drosophila PCNA gene function. | Thacker, SA., et al. 2003. Curr Biol. 13: 53-8. PMID: 12526745
- Germline mutations at microsatellite loci in homozygous and heterozygous mutants for mismatch repair and PCNA genes in Drosophila. | Baida, A., et al. 2003. DNA Repair (Amst). 2: 827-33. PMID: 12826283
- The human proliferating Cell nuclear antigen regulates transcriptional coactivator p300 activity and promotes transcriptional repression. | Hong, R. and Chakravarti, D. 2003. J Biol Chem. 278: 44505-13. PMID: 12937166
- Armadillo/Pangolin regulates PCNA and DREF promoter activities. | Kwon, E., et al. 2004. Biochim Biophys Acta. 1679: 256-62. PMID: 15358517
- GFP-PCNA as an S-phase marker in embryos during the first and subsequent cell cycles. | Kisielewska, J., et al. 2005. Biol Cell. 97: 221-9. PMID: 15584900
- Germline genomic instability in PCNA mutants of Drosophila: DNA fingerprinting and microsatellite analysis. | López, A., et al. 2005. Mutat Res. 570: 253-65. PMID: 15708584
- The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. | Woods, AL., et al. 1991. Histopathology. 19: 21-7. PMID: 1680784
- Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. | Waseem, NH. and Lane, DP. 1990. J Cell Sci. 96 (Pt 1): 121-9. PMID: 1695635
- Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. | Bravo, R. and Macdonald-Bravo, H. 1987. J Cell Biol. 105: 1549-54. PMID: 2889739
| Espèces | Nom du Gène | Gene ID | Localisation chromosomique | Numéro d'accession de l'isoforme (ARNm) | Numéro d'accès aux protéines | Numéro OMIM™ |
|---|---|---|---|---|---|---|
| Human | PCNA | Entrez Gene ID: 5111 | 20p13 | NM_002592, NM_182649 | UniProt ID: P12004 | 176740 |
| Mouse | Pcna | Entrez Gene ID: 18538 | 2 F2 | NM_011045 | UniProt ID: P17918 | N/A |
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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PCNA Antibody (PC10) has 2,909 citations in a variety of scientific publications.
Primary antibodies like Anti-PCNA Antibody (PC10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
What blocking procedure to you recommend for using this mouse monoclonal antibody on mouse tissue?
Thank you for your question. Incubate specimens for 30 minutes with 10% normal blocking serum in PBS .https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Date de publication: 2024-02-29
does antibody m-IgGκ BP-HRP:(sc-516102) compatible to using as secondary antibody against Anti-PCNA Antibody (PC10): sc-56 for western blot
Thank you for your question. Anti-PCNA Antibody (PC10) is a mouse monoclonal IgG2a κ PCNA antibody, so it is compatible with m-IgGκ BP-HRP:(sc-516102), which recognizes kappa light chain mouse IgG, IgA and IgM antibodies.
Date de publication: 2023-04-15
Recently we observed that the PCNA sc-56 antibody in the tube is precipitated. It was stored at 4 degrees as recommended. Can the antibody be used for experimental purpose? If yes how do we use it? any recommendation from company's side?
Thank you for your question. Please contact technical service directly so they can look into this matter.
Date de publication: 2021-01-18
Which antigen retrieval buffer should be used with PCNA sc-56, citrate or Tris? Can you please include this information in the product datasheet so that it becomes convenient for the user to begin with.
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this PCNA antibody. The detailed method can be found in our recommended protocol online.
Date de publication: 2023-04-18
Can I use this mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue?
Thank you for your question. We have not tested this antibody on swine or pig tissue, however, we are aware of a few publications citing use of this antibody in pig samples. For example, PMID: 26389662.
Date de publication: 2019-07-13
We are new to IHC. Thinking of using this antibody, PCNA Antibody: sc-56on our chicken samples. Wondering if there are any preferences on the conjugate for the secondary antibody, such as HRP, biotin etc. We don't need fluorescence
Thank you for your question. This antibody has been previously cited for detection of PCNA in chicken samples. For IHC, you could use the unconjugated antibody, with an appropriate secondary antibody for detection, or the Biotin conjugated primary with an ABC Staining System, or the HRP conjugate for direct immunoperoxidase staining. Using the directly conjugated primary antibody can save you time and money on reagents. We do not have a preference for this particular antibody, and think you would be successful using either version of the antibody, depending on your preferences in your lab.
Date de publication: 2023-04-15
PCNA Antibody (PC10) how much of 200ug/ml of this antibody I am going to get in one order?
Thank you for your question. This is provided as 200 ug in 1 mL.
Date de publication: 2020-01-15
I'm wondering about the region of PCNA that is recognized by this antibody SC-56, PC10? Would an N-terminal Flag tag get in the way? Thank you.
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not been determined. However, some report that this clone (PC10) recognizes an epitope within the amino acid range of 111-125. In that case, we would not expect an N-terminal Flag tag to prevent this antibody from recognizing PCNA that contains this sequence.
Date de publication: 2019-03-21
- Anticorps PCNA (PC10) Questions - page 2
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Rated 5 de 5 de AndreG par Very good antibody The antibody works very well in western blot using 30ug of protein
Date de publication: 2024-04-05
Rated 5 de 5 de xlat par very good despite low dilution needed 1:100 dilution Antigen retrieval: Citrate buffer (pH 6.0) in a microwave
Date de publication: 2024-02-12
Rated 5 de 5 de scientific worker par IHC效果很好 strong and specifical signals in IHC-P experiments，staining mouse tissues.
Date de publication: 2022-11-20
Rated 5 de 5 de 21dhruva par Works great for IF with super-resolution imaging I got the Alexa-488 conjugated antibody and have used to for IF on U-2 OS cells. There was nuclear specific PCNA labelling. I imaged the samples with dSTORM and SIM methods and the immunolabeling looks great.
Date de publication: 2021-07-29
Rated 5 de 5 de KostasS par Works very well! We blotted several times with this antibody and it works perfectly!
Date de publication: 2021-06-16
Rated 5 de 5 de Paloma par PCNA Ab for WB and IF Excellent antibody for WB and IF (fixed with paraformaldehyde) dilution 1:2000 Good housekeeping protein for human colon cancer cell lines
Date de publication: 2021-03-23
Rated 5 de 5 de gkyriakopoulos par Excellent antibody Excellent bands with high dilution and no background
Date de publication: 2021-03-17
Rated 5 de 5 de PAULA C par SIRTs inhibitors and sorafenib SIRTs inhibitors lead to a diminution of cellular proliferation during sorafenib treatment
Date de publication: 2021-02-26
- Anticorps PCNA (PC10) Avis - page 2
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Anticorps PCNA (PC10) est évalué 4.8 de 5 de 113.
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