Phospho-Btk (Tyr551) (G12) rabbit mAb Biotin conjugate Antibody
Référence orb1946086-10tests
Conditionnement : 10tests
Marque : Biorbyt
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Phospho-Btk (Tyr551) (G12) rabbit mAb Biotin conjugate Antibody
Catalog Number: orb1946086
| Catalog Number | orb1946086 |
|---|---|
| Category | Antibodies |
| Description | Phospho-Btk (Tyr551) (G12) rabbit mAb Biotin conjugate Antibody |
| Clonality | Monoclonal |
| Clone Number | BtkY551-G12 |
| Tested applications | ELISA, FC |
| Reactivity | Human |
| Isotype | Rabbit IgGk |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding human Btk (Y551) |
| Dilution range | For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Conjugation | Biotin |
| UniProt ID | Q06187 |
| Storage | 2-8°C |
| Buffer/Preservatives | 1X PBS, 0.09% NaN3, 0.2% rAlbumin |
| Alternative names | Bruton tyrosine kinase, Tyrosine-protein kinase BT |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Btk (Tyr551) antibody BtkY551-G12-Biotin conjugate at 0.1 μg/mL. Cat. #orb1946086.
Peptide ELISA using BtkY551-G12-Biotin conjugate (Cat. #orb1946086) titered starting from 0.3 ug/mL shows binding to only Btk pY551 phospho peptide and no cross-reactivity to Btk Y551 non-phospho peptide or to control peptides.
Direct ELISA using Ramos cellular lysate coated directly to the plate surface after lysis following no treatment or treatment with 1mM pervanadate for 30 min. ELISA wells were tested in duplicate using 0.12 mg/mL total protein coated lysate and 1 ug/mL BtkY551-G12-Biotin conjugate (Cat. #orb1946086) IgG.