PHR1 | Phosphate starvation response 1 (monocots)

Référence AS184222

Conditionnement : 50µg

Marque : Agrisera

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AS18 4222   | Clonality: Polyclonal  |  Host: Rabbit | Reactivity: Hordeum vulgare

PHR1 | Phosphate starvation response 1 (monocots) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS18 4222)
PHR1 | Phosphate starvation response 1 (monocots)



DATA SHEET IN PDF


Product Information

Immunogen Contact us
Not reactive in dicots

Application examples

Application examples Application example


Western blot using anti-Hordeum vulgare PHR1 antibodies


Samples: purified MBP tag protein, 50 ng, (1), purified MBP-HvTF (MYB-family like PHR1) tag protein 50 ng, (2), purified MBP+HvPHR1, 50 ng, (3).  Samples were separated on 13% SDS-PAGE and blotted 1h to PVDF/nitrocellulose using semi-dry transfer. Blot was blocked with 10% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:50 000 in 10% milk with TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with chemiluminescent detection reagent. Exposure time was 3 minutes.

Courtesy of Msc. Paweł Sega, Institute of Molecular Biology and Biotechnology, Department of Gene Expression, UAM, Poznań, Poland

Additional information

Background

Background PHR1 (Phosphate starvation response 1) is nuclear localized and involved in transcription regulation.

Product citations

immunogen: Contact us
not reactive in: dicots
Picture (footer): Application example


Western blot using anti-Hordeum vulgare PHR1 antibodies


Samples: purified MBP tag protein, 50 ng, (1), purified MBP-HvTF (MYB-family like PHR1) tag protein 50 ng, (2), purified MBP+HvPHR1, 50 ng, (3).  Samples were separated on 13% SDS-PAGE and blotted 1h to PVDF/nitrocellulose using semi-dry transfer. Blot was blocked with 10% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:50 000 in 10% milk with TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with chemiluminescent detection reagent. Exposure time was 3 minutes.

Courtesy of Msc. Paweł Sega, Institute of Molecular Biology and Biotechnology, Department of Gene Expression, UAM, Poznań, Poland

background: PHR1 (Phosphate starvation response 1) is nuclear localized and involved in transcription regulation.