Pol II (8WG16) AC

Pol II Antibody (8WG16) is an IgG2a κ mouse monoclonal Pol II antibody (also designated Pol II antibody or RNA Polymerase II antibody) that detects the highly conserved heptapeptide repeat of the largest subunit of eukaryotic Pol II of mouse, rat and human origin by WB, IP and IF. Pol II Antibody (8WG16) is available as the non-conjugated anti-Pol II antibody form, as well as multiple conjugated forms of anti-Pol II antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.  |  Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
2. Structure and function of RNA polymerase II.  |  Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
3. Mechanism of RNA polymerase I transcription.  |  Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
4. The RNA polymerase II transcription cycle: cycling through chromatin.  |  Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
5. RNA polymerase III transcription and cancer.  |  White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
6. BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
7. Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity.  |  Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
8. Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein.  |  Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
9. Downstream DNA selectively affects a paused conformation of human RNA polymerase II.  |  Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pol II Antibody (8WG16) has 91 citations in a variety of scientific publications.

Primary antibodies like Anti-Pol II Antibody (8WG16) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.