Polyoma virus middle T antigen (PyMT) Alexa Fluor® 647
Référence sc-53481 AF647
Conditionnement : 200µg/ml
Marque : Santa Cruz Biotechnology
Anticorps Polyoma virus middle T antigen (PyMT): sc-53481
- L'anticorps Polyoma virus middle T antigen PyMT est un monoclonal IgG2b de rat, cité dans 19 publications, fourni en 200 µg/ml
- raised against Polyoma virus-transformed Wistar rat fibroblast cell line REWA5/T1A1
- L'Anticorps Polyoma virus middle T antigen (PyMT) est recommandé pour la détection de Polyoma Virus par IP et IF
- Anti-L'Anticorps Polyoma virus middle T antigen (PyMT) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Polyoma virus middle T antigen Antibody (PyMT). This work is in progress.
ACCÈS RAPIDE AUX LIENS
and Cruz Marker™ MW Standards Promotional
Polyoma virus middle T antigen Antibody (PyMT) is an IgG2b rat monoclonal Polyoma virus middle T antigen antibody that detects Polyoma virus- middle T antigen of Polyoma Virus origin by IP and IF. Polyoma virus middle T antigen Antibody (PyMT) is available as the non-conjugated anti-Polyoma virus middle T antigen antibody form, as well as multiple conjugated forms of anti-Polyoma virus middle T antigen antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The polyoma virus (Py) is a small oncogenic DNA virus that belongs to the family Polymaviridae and produces multiple tumors in the infected host. Py encodes three early proteins: large, middle and small T (tumor) antigen. Polyoma virus large T antigen (PyLT) is a nuclear phosphoprotein that helps to regulate viral replication and gene expression, allows isolation of viral T antigens, and can induce cellular DNA replication in the absence of other virus-transforming genes. Polyoma virus middle T antigen (PyMT) contains 421 amino acids and is divided into at least three domains, some of which are shared with PyLT and Polyoma virus small T antigen (PyST). PyMT is a major transforming protein responsible for inducing the phenotype of transformed cells and, without it, transformation does not occur. PyST functions in transformation and in productive infection.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Expression of biologically active middle T antigen of polyoma virus from recombinant baculoviruses. | Forstová, J., et al. 1989. Nucleic Acids Res. 17: 1427-43. PMID: 2646594
- Expression of polyoma early gene products in E. coli. | Schaffhausen, B., et al. 1985. Nucleic Acids Res. 13: 501-19. PMID: 2987799
- Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts. | Jat, PS. and Sharp, PA. 1986. J Virol. 59: 746-50. PMID: 3016337
- Polyomavirus small T antigen enhances replication of viral genomes in 3T6 mouse fibroblasts. | Berger, H. and Wintersberger, E. 1986. J Virol. 60: 768-70. PMID: 3022009
- Binding of polyomavirus large T antigen to the human hsp70 promoter is not required for trans activation. | Kingston, RE., et al. 1986. Mol Cell Biol. 6: 3180-90. PMID: 3023966
- Monoclonal antibodies against polyoma virus tumor antigens. | Dilworth, SM. and Griffin, BE. 1982. Proc Natl Acad Sci U S A. 79: 1059-63. PMID: 6175960
- Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen. | Dilworth, SM. 1982. EMBO J. 1: 1319-28. PMID: 6327264
- The polyoma virus T antigen interferes with interferon-inducible gene expression. | Weihua, X., et al. 1998. Proc Natl Acad Sci U S A. 95: 1085-90. PMID: 9448289
- Polyomavirus large T antigen binds the transcriptional coactivator protein p300. | Nemethova, M. and Wintersberger, E. 1999. J Virol. 73: 1734-9. PMID: 9882390
Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Polyoma virus middle T antigen Antibody (PyMT) has 19 citations in a variety of scientific publications.
Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
Hi, How can we obtain the free 10 ug sample to test if this antibody will work for us? Thanks for your time.
What is the exact epitope recognized by Polyoma virus middle T antigen (PyMT): sc-53481 monoclonal antibody?
- y_2024, m_5, d_6, h_4CST
- bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
- cp_1, bvpage1
- co_hasquestionsanswers, tq_2
- loc_fr_FR, sid_53481, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
- clientName_scbt
- bvseo_sdk, java_sdk, bvseo-4.0.0
- CLOUD, getContent, 108ms
- QUESTIONS, PRODUCT
- y_2024, m_5, d_6, h_4
- bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
- cp_1, bvpage1
- co_hasreviews, tv_1, tr_4
- loc_fr_FR, sid_53481, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
- clientName_scbt
- bvseo_sdk, java_sdk, bvseo-4.0.0
- CLOUD, getReviews, 20ms
- REVIEWS, PRODUCT
- y_2024, m_5, d_6, h_4
- bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
- cp_1, bvpage1
- co_hasreviews, tv_1, tr_4
- loc_fr_FR, sid_53481, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
- clientName_scbt
- bvseo_sdk, java_sdk, bvseo-4.0.0
- CLOUD, getAggregateRating, 98ms
- REVIEWS, PRODUCT