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> PUMA (NT) Antibody

PUMA (NT) Antibody

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Référence ASM10417-P594-100

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  • IHC - PUMA (NT) Antibody ASM10417
    Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (ASM10417). Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10417) at 1:100.
  • WB - PUMA (NT) Antibody ASM10417
    Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (ASM10417). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10417) at 1:500.
  • IHC - PUMA (NT) Antibody ASM10417
    Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (ASM10417). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10417) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9BXH2
Other Accession NP_001120712.1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Description Rabbit Anti-Human PUMA (NT) Polyclonal
Target/Specificity Detects the N-terminal domain of PUMA ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
Other Names BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Immunogen N-terminal amino acids of human PUMA
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 0.02% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 2 µg/ml of SPC-165 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion