Rabbit IgM (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ID, IP
Recommended Dilution	Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in rabbit serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rabbit IgM from serum or plasma. Recommended dilutions: Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum. Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Highly purified normal IgM isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal rabbit serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. Cross-reactivity: The antiserum does not cross-react with any other component of the rabbit Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	State: Serum State: Lyophilized (Delipidated and heat inactivated) stable whole antiserum - No preservative added
Form	Serum
Reconstitution Method	Restore by adding 1,0 ml sterile distilled water. Dilutions may be prepared by adding PBS, pH 7.2
Concentration	Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Conjugation	Unconjugated
Storage	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.