Rabbit IgM (mu) Antibody Peroxidase Conjugated

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommended Dilution	WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:20,000
Reactivities	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Anti-Rabbit IgM (mu) was produced by repeated immunization with rabbit IgM mu heavy chain in goat.
Specificity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgM and Rabbit Serum. No reaction was observed against Rabbit IgG.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
Concentration	10.0 mg/mL - lot specific
Conjugation	HRP
Storage	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Note	Rabbit IgM (mu) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Rabbit IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.