Raf-B Antibody (F-7) is an IgG_2a κ mouse monoclonal Raf-B antibody (also designated BRAF antibody or RAFB antibody) that detects the Raf-B protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Raf-B Antibody (F-7) is available as both the non-conjugated anti-Raf-B antibody form, as well as multiple conjugated forms of anti-Raf-B antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Expression of raf family proto-oncogenes in normal mouse tissues.  |  Storm, SM., et al. 1990. Oncogene. 5: 345-51. PMID: 1690378
2. Protein kinase cascades in meiotic and mitotic cell cycle control.  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
3. Mutational activation of c-raf-1 and definition of the minimal transforming sequence.  |  Heidecker, G., et al. 1990. Mol Cell Biol. 10: 2503-12. PMID: 2188091
4. Expression of the c-raf protooncogene in human hematopoietic cells and cell lines.  |  Sariban, E., et al. 1987. Blood. 69: 1437-40. PMID: 2436688
5. Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity.  |  Morrison, DK., et al. 1988. Proc Natl Acad Sci U S A. 85: 8855-9. PMID: 3057494
6. Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.  |  Ray, LB. and Sturgill, TW. 1988. Proc Natl Acad Sci U S A. 85: 3753-7. PMID: 3287375
7. Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.  |  Huleihel, M., et al. 1986. Mol Cell Biol. 6: 2655-62. PMID: 3491291
8. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus.  |  Rapp, UR., et al. 1983. Proc Natl Acad Sci U S A. 80: 4218-22. PMID: 6308607

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BRAF Antibody (F-7) has 283 citations in a variety of scientific publications.

Primary antibodies like Anti-BRAF Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.