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> Rat IgA (alpha chain) antibody (Biotin)

Rat IgA (alpha chain) antibody (Biotin)

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Référence orb347835-1mg

Conditionnement : 1mg

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Rat IgA (alpha chain) antibody (Biotin)

Rat IgA (alpha chain) antibody (Biotin)

Catalog Number: orb347835

Catalog Numberorb347835
CategoryAntibodies
DescriptionRat IgA (alpha chain) antibody (Biotin)
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityRat
IsotypeIgG
ImmunogenRat IgA alpha heavy chain
Concentration1.0 mg/mL
Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/AppearanceLyophilized
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against other Rat heavy or light chain proteins.
ConjugationBiotin
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesGoat anti-Rat IgA (alpha chain) biotin Conjugated
NoteFor research use only
Application notesAnti-Rat IgA Biotin Conjugated Antibody has been tested by western blot and assayed against 1.0 ug of Rat IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Expiration Date12 months from date of receipt.