Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody

Specifications

Product Data
Recommended Dilution	Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/2,000-1/10,000. Immunofluorescence microscopy: 1/1,000-1/5,000.
Reactivities	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Rat IgG, IgA and IgM whole molecules.
Specificity	This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM. This reagent is suitable for the detection of all Rat isotypes and chain combinations.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Label: FITC State: Lyophilized purified Ig fraction. Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 3.5 moles FITC per mole of Goat IgG.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity chromatography.
Conjugation	FITC
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.