Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommended Dilution	ELISA: 1/100,000. Western blot: 1/500-1/3,000. Immunohistochemistry: 1/500-1/1,500.
Reactivities	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Rat IgG, IgA, IgM whole molecules
Specificity	This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Rat antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Thimerosal Label: Horseradish Peroxidase.  Conjugation Chemistry: Periodate
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity Chromatography
Conjugation	HRP
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.