Rat IgE (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ID, IP
Recommended Dilution	This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgE in Rat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Rat IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitin Titre: 1/32 when tested against appropriate concentrations of rat IgE in agar-block immunodiffusion titration.
Reactivities	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Highly purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is restricted to the Fc part of the IgE molecule, which represents strict isotype (class) specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the immunogen a single precipitin line is obtained which shows a reaction of full identity with the precipitin lines obtained against rat serum and ascites containing monoclonal IgE. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. Cross-reactivity: The antiserum does not cross-react with any other component of the rat immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, because of the very low level of IgE in most species.
Formulation	State: Serum State: Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives.
Form	Serum
Reconstitution Method	Restore by adding 1 ml of sterile distilled water.
Concentration	Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.